|
KMID : 0613820060160071235
|
|
Journal of Life Science
2006 Volume.16 No. 7 p.1235 ~ p.1242
|
|
|
Modulation of Cell Cycle Regulators by Sulforaphane in Human Hepatocarcinoma HepG2 Cells
|
|
Bae Song-Ja
Kim Ki-Young
Yoo Young-Hyun
Choi Byung-Tae
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Sulforaphane, an isothiocyanate derived from hydrolysis of glucoraphanin in broccoli and other cruciferous vegetables, was shown to induce phase II detoxification enzymes and inhibit chemically induced mammary tumors in rodents. Recently, sulforaphane is known to induce cell cycle arrest and apoptosis in human cancer cells, however its molecular mechanisms are poorly understood. In the present study, we demonstrated that sulforaphane acted to inhibit proliferation and induce morphological changes of human hepatocarcinoma HepG2 cells. Treatment of HepG2 cells with 10 μM or 15 μM sulforaphane resulted in significant G2/M cell cycle arrest as determined by DNA flow cytometry. Moreover, 20 μM sulforaphane significantly induced the population of sub-G1 cells suggesting that sulforaphane induced apoptosis. This anti-proliferative effect of sulforaphane was accompanied by a marked inhibition of cyclin A, cyclin B1 and Cdc2 protein. However, the levels of tumor suppressor p53 and Cdk inhibitor p21 mRNA and protein expression were significantly increased by sulforaphane treatment in a concentration-dependent manner. Although further studies are needed, the present work suggests that sulforaphane may be a potential chemoprevetive/chemotherapeutic agent for the treatment of human cancer cells.
|
|
|
KEYWORD
|
|
|
Sulforaphane, HepG2 cells, cell cycle, p53, p21
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|